TGF alpha Protein, Human, Recombinant

The miR-137 served as a tumor suppressor in non-small cell lung cancer (NSCLC) and its suppressive effect is mediated by repressing TGFA expression. TGFA gene expression was significantly higher in tumor tissues compared to adjacent normal tissue and high TGFA gene expression strongly correlated with poor survival in patients with lung adenocarcinoma, and miR-374a suppresses lung adenocarcinoma cell proliferation and invasion via targeting TGFA gene expression. Transforming growth factor alpha (TGFA) is a well-characterized mammalian growth factor that might contribute to the development of Cleft lip and palate (CL/P).

Pack Size	Availability	Price/USD
20 μg	In stock	$ 165.00
100 μg	5 days	$ 462.00
1 mg	5 days	$ 3,000.00

Pack Size

Availability

Price/USD

Quantity

20 μg

In stock

$ 165.00

100 μg

5 days

$ 462.00

1 mg

5 days

$ 3,000.00

Biological Information

1.Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 μg/ml (100 μl/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL. 2. Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.

Description

Species	Human
Expression System	E. coli
Tag	Tag Free
Accession Number	P01135-1
Synonyms	transforming growth factor α, transforming growth factor alpha, TGFa, TGF-α, TFGA
Construction	A DNA sequence encoding the human TGFa (P01135-1) (Val40-Ala89) was expressed and purified.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 5.6 kDa

Endotoxin	Please contact us for more information.
Formulation	Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The miR-137 served as a tumor suppressor in non-small cell lung cancer (NSCLC) and its suppressive effect is mediated by repressing TGFA expression. TGFA gene expression was significantly higher in tumor tissues compared to adjacent normal tissue and high TGFA gene expression strongly correlated with poor survival in patients with lung adenocarcinoma, and miR-374a suppresses lung adenocarcinoma cell proliferation and invasion via targeting TGFA gene expression. Transforming growth factor alpha (TGFA) is a well-characterized mammalian growth factor that might contribute to the development of Cleft lip and palate (CL/P).

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TGF alpha Protein, Human, Recombinant

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