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Anti-ECH1 Antibody (4B261)

😃Good
Catalog No. TMAY-02748
Alias HPXEL, enoyl CoA hydratase 1, peroxisomal

Anti-ECH1 Antibody (4B261) is a Rabbit antibody targeting ECH1. Anti-ECH1 Antibody (4B261) can be used in WB,ELISA.

Anti-ECH1 Antibody (4B261)

Anti-ECH1 Antibody (4B261)

😃Good
Catalog No. TMAY-02748Alias HPXEL, enoyl CoA hydratase 1, peroxisomal
Anti-ECH1 Antibody (4B261) is a Rabbit antibody targeting ECH1. Anti-ECH1 Antibody (4B261) can be used in WB,ELISA.
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100 μL$270 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ECH1 Antibody (4B261) is a Rabbit antibody targeting ECH1. Anti-ECH1 Antibody (4B261) can be used in WB,ELISA.
AliasHPXEL, enoyl CoA hydratase 1, peroxisomal
Ig Type
Monoclonal Rabbit IgG
Clone
4B261
Reactivity
Human
Verified Activity
Anti-ECH1 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: Jurkat Whole Cell Lysate.

-Lane C: A549 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:36 kDa.

-Observed band size:36 kDa
Application
WB,ELISA
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human ECH1 (rh ECH1; TMPY-03464; Q13011; Thr34-Leu328).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ECH1 Protein (TMPY-03464)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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