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Anti-ECH1 Antibody (4B261) is a Rabbit antibody targeting ECH1. Anti-ECH1 Antibody (4B261) can be used in ELISA,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $270 | 7-10 days | 7-10 days |
| Description | Anti-ECH1 Antibody (4B261) is a Rabbit antibody targeting ECH1. Anti-ECH1 Antibody (4B261) can be used in ELISA,WB. |
| Synonyms | HPXEL, enoyl CoA hydratase 1, peroxisomal |
| Ig Type | Rabbit IgG |
| Clone | 4B261 |
| Reactivity | Human |
| Specificity | Human ECH1 |
| Verified Activity | Anti-ECH1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: Jurkat Whole Cell Lysate. -Lane C: A549 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:36 kDa. -Observed band size:36 kDa |
| Application | |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ECH1 (rh ECH1; TMPY-03464; Q13011; Thr34-Leu328). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ECH1 is a member of the hydratase/isomerase superfamily. ECH1 shows high sequence similarity to enoyl-CoA hydratases of several species, particularly within a conserved domain characteristic of these proteins. ECH1 contains a C-terminal peroxisomal targeting sequence and localizes to peroxisomes. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. ECH1 functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ECH1 Protein (TMPY-03464) |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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