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Anti-Alpha-fetoprotein Antibody (9O812) (Synonyms: α-fetoprotein, HPAFP, FETA, Alpha-fetoprotein, AFPD)

Catalog No. TMAY-02268 Copy Product Info
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Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in ELISA,IP,WB.

Anti-Alpha-fetoprotein Antibody (9O812)

Copy Product Info
🥰Excellent
Catalog No. TMAY-02268
Synonyms α-fetoprotein, HPAFP, FETA, Alpha-fetoprotein, AFPD

Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in ELISA,IP,WB.

Anti-Alpha-fetoprotein Antibody
(9O812)
Pack SizePriceUSA StockGlobal StockQuantity
20 μL$947-10 days7-10 days
100 μL$2697-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in ELISA,IP,WB.
Synonymsα-fetoprotein, HPAFP, FETA, Alpha-fetoprotein, AFPD
Ig Type
Rabbit IgG
Clone
9O812
Reactivity
Human
Specificity
Human Alpha-fetoprotein
Verified Activity
1. AFP was immunoprecipitated using:
-Lane A:0.5 mg HepG2 Whole Cell Lysate.
-2 µL anti-AFP rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
-Primary antibody:
-Anti-AFP rabbit monoclonal antibody, at 1:100 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 68 kDa.
-Observed band size: 68 kDa.
2. Anti-AFP rabbit monoclonal antibody at 1:500 dilution.
-Lane A: HepG2 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:69 kDa.
-Observed band size:69 kDa
Application
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; TMPY-02509; P02771; Met1-Val609).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundAlpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human AFP / alpha-fetoprotein protein (TMPY-02509)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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