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Anti-Alpha-fetoprotein Antibody (9O812)
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Catalog No. TMAY-02268
Alias α-fetoprotein, HPAFP, FETA, Alpha-fetoprotein, AFPD
Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in WB,ELISA,IP.
Anti-Alpha-fetoprotein Antibody (9O812)
Anti-Alpha-fetoprotein Antibody (9O812)
😃Good
Catalog No. TMAY-02268Alias α-fetoprotein, HPAFP, FETA, Alpha-fetoprotein, AFPD
Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $94 | 7-10 days | |
| 100 μL | $269 | 7-10 days |
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Antigen Details
Chemical Properties
| Description | Anti-Alpha-fetoprotein Antibody (9O812) is a Rabbit antibody targeting Alpha-fetoprotein. Anti-Alpha-fetoprotein Antibody (9O812) can be used in WB,ELISA,IP. |
| Alias | α-fetoprotein, HPAFP, FETA, Alpha-fetoprotein, AFPD |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 9O812 |
| Reactivity | Human |
| Verified Activity | 1. AFP was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -2 µL anti-AFP rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-AFP rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 68 kDa. -Observed band size: 68 kDa. 2. Anti-AFP rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:69 kDa. -Observed band size:69 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human AFP / alpha-fetoprotein (rh AFP / alpha-fetoprotein; TMPY-02509; P02771; Met1-Val609). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human AFP / alpha-fetoprotein protein (TMPY-02509) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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