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Anti-MAP1LC3B Antibody (3M54)
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Catalog No. TMAH-00723
Alias Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 lig, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B
Anti-MAP1LC3B Antibody (3M54) is an antibody targeting MAP1LC3B. Anti-MAP1LC3B Antibody (3M54) can be used in ELISA, WB, FC.
Anti-MAP1LC3B Antibody (3M54)
Anti-MAP1LC3B Antibody (3M54)
😃Good
Catalog No. TMAH-00723Alias Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 lig, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B
Anti-MAP1LC3B Antibody (3M54) is an antibody targeting MAP1LC3B. Anti-MAP1LC3B Antibody (3M54) can be used in ELISA, WB, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Chemical Properties
| Description | Anti-MAP1LC3B Antibody (3M54) is an antibody targeting MAP1LC3B. Anti-MAP1LC3B Antibody (3M54) can be used in ELISA, WB, FC. |
| Alias | Microtubule-associated proteins 1A/1B light chain 3B, Microtubule-associated protein 1 lig, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, MAP1 light chain 3-like protein 2, Autophagy-related ubiquitin-like modifier LC3 B, Autophagy-related protein LC3 B |
| Ig Type | Rabbit IgG |
| Clone | 3M54 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,RAW264.7 whole cell lysate,U251 whole cell lysate,Hela whole cell lysate -All lanes: LC3B antibody at 1:500 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 15 kDa -Observed band size: 15 kDa 2. Overlay Peak curve showing Hela cells stained with TMAH-00723 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, FC |
| Recommended Dose | WB:1:500-1:2000; FC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human MAP1LC3B |
| Antigen Species | Human |
| Gene ID | 81631 |
| Uniprot ID | |
| Biology Area | Neuroscience, Cancer, Cardiovascular, Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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