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TOPBioactivityRelated Conjugates and FormulationsAntigen DetailsChemical infoStorage&SolubilityCalculator

Anti-Phospho-RAF1 (Ser43) Antibody (8R108)

😃Good
Catalog No. TMAH-00954
Alias RAF1 (p-Ser43), RAF1 (p-S43), p-RAF1 (Ser43), p-RAF1 (S43), Phospho-RAF1 (S43)

Anti-Phospho-RAF1 (Ser43) Antibody (8R108) is an antibody targeting Phospho-RAF1 (Ser43). Anti-Phospho-RAF1 (Ser43) Antibody (8R108) can be used in ELISA, IF.

Anti-Phospho-RAF1 (Ser43) Antibody (8R108)

Anti-Phospho-RAF1 (Ser43) Antibody (8R108)

😃Good
Catalog No. TMAH-00954Alias RAF1 (p-Ser43), RAF1 (p-S43), p-RAF1 (Ser43), p-RAF1 (S43), Phospho-RAF1 (S43)
Anti-Phospho-RAF1 (Ser43) Antibody (8R108) is an antibody targeting Phospho-RAF1 (Ser43). Anti-Phospho-RAF1 (Ser43) Antibody (8R108) can be used in ELISA, IF.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$347 7-10 days
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Instruction Manual of Detection Antibody
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IF

Reactivity: Human
AliasRAF1 (p-Ser43), RAF1 (p-S43), p-RAF1 (Ser43), p-RAF1 (S43), Phospho-RAF1 (S43)
Ig Type
Rabbit IgG
Clone
8R108
Reactivity
Human
Verified Activity
Immunofluorescence staining of Hela with TMAH-00954 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application
ELISA, IF
Recommended Dose
IF:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundSerine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Phospho-RAF1 (S43)
Antigen Species
Human
Gene ID
5894
Uniprot ID
Biology Area
Signal Transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

RAF1 (pSer43)RAF1 (pS43)RAF1 (phospho-Ser43)RAF1 (phospho-S43)Raf-1RAF1RAF proto-oncogene serine/threonine-protein kinaseRAF 1RAFProto-oncogene c-RAF (cRaf)pRAF1 (Ser43)pRAF1 (S43)c-RafcRaf
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