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Anti-MMAE Antibody (2O521) is a Mouse antibody targeting MMAE. Anti-MMAE Antibody (2O521) can be used in ELISA.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 20 μg | $92 | 7-10 days | 7-10 days | |
| 100 μg | $292 | 7-10 days | 7-10 days |
| Description | Anti-MMAE Antibody (2O521) is a Mouse antibody targeting MMAE. Anti-MMAE Antibody (2O521) can be used in ELISA. |
| Ig Type | Mouse IgG1 |
| Clone | 2O521 |
| Reactivity | other |
| Specificity | Specifically recognize MMAE. No cross-reactivity with MMAF. No cross-reactivity in ELISA with MMAF |
| Verified Activity | 1. Immobilized MMAE-ADC, Naked Antibody, MMAE-OVA, MMAF-OVA, OVA, at 1 μg/mL (100 μL/well), then add the Anti-MMAE Antibody to detect the cross-reactivity. (Validation Experiment) 2. Immobilized Anti-MMAE Antibody at 2 μg/mL (100 μL/well) can bind the MMAE-ADC, the EC50 is 50-1000 ng/mL. The naked antibody (without MMAE) is the negative control. 3. Immobilized Anti-MMAE Antibody at 2 μg/mL (100 μL/well), gradient-diluted free MMAE from 10 μg/ml (50 μL/well) blocked the binding of MMAE Antibody to MMAE-HRP 2 μg/mL (50 μL/well). (Validation Experiment) |
| Application | |
| Recommended Dose | ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with MMAE conjugated to BSA. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjucates | Unconjugated |
| Immunogen | MMAE conjugated to BSA |
| Antigen Species | other |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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