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Anti-MMAE Antibody (2O521) is a Mouse antibody targeting MMAE. Anti-MMAE Antibody (2O521) can be used in ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $92 | 7-10 days | |
100 μg | $292 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: ELISA Reactivity: other |
Ig Type | Mouse IgG1 |
Clone | 2O521 |
Reactivity | other |
Verified Activity | 1. Immobilized MMAE-ADC, Naked Antibody, MMAE-OVA, MMAF-OVA, OVA, at 1 μg/mL (100 μL/well), then add the Anti-MMAE Antibody to detect the cross-reactivity. (Validation Experiment) 2. Immobilized Anti-MMAE Antibody at 2 μg/mL (100 μL/well) can bind the MMAE-ADC, the EC50 is 50-1000 ng/mL. The naked antibody (without MMAE) is the negative control. 3. Immobilized Anti-MMAE Antibody at 2 μg/mL (100 μL/well), gradient-diluted free MMAE from 10 μg/ml (50 μL/well) blocked the binding of MMAE Antibody to MMAE-HRP 2 μg/mL (50 μL/well). (Validation Experiment) |
Application | ELISA |
Recommended Dose | ELISA: 1:1000-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with MMAE conjugated to BSA. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Conjucates | Unconjugated |
Immunogen | MMAE conjugated to BSA |
Antigen Species | other |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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