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Anti-HSPD1 Antibody (8M190)
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Catalog No. TMAH-00582
Alias P60 lymphocyte protein, Mitochondrial matrix protein P1, HuCHA60, HSPD 1, HSP-60, HSP60, Heat shock protein 60, EC 3.6.4.9, CPN60, Chaperonin 60, 60 kDa heat shock protein, 60 kDa chaperonin
Anti-HSPD1 Antibody (8M190) is an antibody targeting HSPD1. Anti-HSPD1 Antibody (8M190) can be used in ELISA, WB, IHC, IP.
Anti-HSPD1 Antibody (8M190)
Anti-HSPD1 Antibody (8M190)
😃Good
Catalog No. TMAH-00582Alias P60 lymphocyte protein, Mitochondrial matrix protein P1, HuCHA60, HSPD 1, HSP-60, HSP60, Heat shock protein 60, EC 3.6.4.9, CPN60, Chaperonin 60, 60 kDa heat shock protein, 60 kDa chaperonin
Anti-HSPD1 Antibody (8M190) is an antibody targeting HSPD1. Anti-HSPD1 Antibody (8M190) can be used in ELISA, WB, IHC, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, IHC, IP Reactivity: Human, Mouse |
| Alias | P60 lymphocyte protein, Mitochondrial matrix protein P1, HuCHA60, HSPD 1, HSP-60, HSP60, Heat shock protein 60, EC 3.6.4.9, CPN60, Chaperonin 60, 60 kDa heat shock protein, 60 kDa chaperonin |
| Ig Type | Rabbit IgG |
| Clone | 8M190 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, Mouse brain tissue -All lanes: HSPD1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 62, 18 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00582 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00582 diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunoprecipitating HSPD1 in HepG2 whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00582 in HepG2 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00582(3µg)+ HepG2 whole cell lysate(500µg) -Lane 3: HepG2 whole cell lysate (10µg) |
| Application | ELISA, WB, IHC, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Mitochondrion matrix. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Hsp60 |
| Antigen Species | Human |
| Gene ID | 3329 |
| Uniprot ID | |
| Biology Area | Isotype/Loading Controls, Tags & Cell Markers, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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