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Anti-LYPD3 Antibody (5Y943)
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Catalog No. TMAY-02083
Alias LY6/PLAUR domain containing 3, C4.4A
Anti-LYPD3 Antibody (5Y943) is a Rabbit antibody targeting LYPD3. Anti-LYPD3 Antibody (5Y943) can be used in ELISA,FCM,ICC/IF.
Anti-LYPD3 Antibody (5Y943)
Anti-LYPD3 Antibody (5Y943)
😃Good
Catalog No. TMAY-02083Alias LY6/PLAUR domain containing 3, C4.4A
Anti-LYPD3 Antibody (5Y943) is a Rabbit antibody targeting LYPD3. Anti-LYPD3 Antibody (5Y943) can be used in ELISA,FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $93 | 7-10 days | |
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, FCM, ICC/IF Reactivity: Human |
| Alias | LY6/PLAUR domain containing 3, C4.4A |
| Ig Type | Rabbit IgG |
| Clone | 5Y943 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane. 2. Flow cytometric analysis of Human LYPD3 expression on PC-3 cells. Cells were stained with purified anti-Human LYPD3, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells. 3. Anti-LYPD3 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:36 kDa. -Observed band size:36 kDa |
| Application | ELISA,FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human C4.4A / LYPD3 (rh C4.4A / LYPD3; TMPY-01859; NP_055215.2; Met 1-His 286). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Ly6 / PLAUR domain-containing protein 3, also known as GPI-anchored metastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein, MIG-C4, and LYPD3, is a cell membrane protein that contains two UPAR/Ly6 domains. Human LYPD3 contains two UPAR/Ly6 domains. LYPD3 is expressed in the placenta, skin, and urothelium. It is found in suprabasal keratinocytes of chronic wounds. Weak expression of LYPD3 is found in the esophagus and peripheral blood mononuclear cells. It is found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression of LYPD3 is found in the tumor component of some noninvasive superficial lesions and invasive and metastatic urothelial cancers. LYPD3 is up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds. LYPD3 supports cell migration. It may be involved in urothelial cell-matrix interactions. It may also be involved in tumor progression |
| Conjucates | Unconjugated |
| Others Formats | APC/PE |
| Antibody Types Available | 3 |
| Immunogen | Recombinant Protein: Human C4.4A / LYPD3 protein (TMPY-01859) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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