Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human

hIgG1

1A889

Human

1. Western Blot
-Positive WB detected in:hela whole cell lysate (20µg), HT29 whole cell lysate (20µg)
-All lanes: FGFR2 antibody at 1:1000
-Secondary: Goat polyclonal to human IgG at 1/40000 dilution
-Predicted band size:92.03, 86.13, 76.70, 28.29, 40.61, 79 kDa
-Observed band size:41 kDa
-Exposure time:120s
2. IHC image of TMAH-00432 diluted at 1:100 and staining in paraffin-embedded lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-00432 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB."

ELISA, WB, IHC

WB:1:500-1:2000; IHC:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Human FGFR2 protein

Human

2263

Neuroscience, Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells