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Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40)
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Catalog No. TMAH-00917
Alias Phospho-MAPK3 (T202) + MAPK1 (T185)
Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40) is an antibody targeting Phospho-MAPK3 (Thr202) + MAPK1 (Thr185). Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40) can be used in ELISA, WB, IHC, IF.
Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40)
Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40)
😃Good
Catalog No. TMAH-00917Alias Phospho-MAPK3 (T202) + MAPK1 (T185)
Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40) is an antibody targeting Phospho-MAPK3 (Thr202) + MAPK1 (Thr185). Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40) can be used in ELISA, WB, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Chemical Properties
| Description | Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40) is an antibody targeting Phospho-MAPK3 (Thr202) + MAPK1 (Thr185). Anti-Phospho-MAPK3 (Thr202) + MAPK1 (Thr185) Antibody (6C40) can be used in ELISA, WB, IHC, IF. |
| Alias | Phospho-MAPK3 (T202) + MAPK1 (T185) |
| Ig Type | Rabbit IgG |
| Clone | 6C40 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in A549 whole cell lysate,HepG2 whole cell lysate(treated with EGF or not) -All lanes Phospho-MAPK3 antibody at 2.35μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 42 KDa -Observed band size: 42 KDa 2. IHC image of TMAH-00917 diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. Immunofluorescence staining of Hela cells with TMAH-00917 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IHC, IF |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. Membrane, caveola. Cell junction, focal adhesion. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-MAPK3 (T202) + MAPK1 (T185) |
| Antigen Species | Human |
| Gene ID | 5595 |
| Uniprot ID | |
| Biology Area | Neuroscience |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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