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Anti-ASGR1 Antibody (8E417)
😃Good
Catalog No. TMAY-01152
Alias HL-1, CLEC4H1, asialoglycoprotein receptor 1, ASGPR1, ASGPR
Anti-ASGR1 Antibody (8E417) is a Rabbit antibody targeting ASGR1. Anti-ASGR1 Antibody (8E417) can be used in WB,ELISA(Cap),IP.
Anti-ASGR1 Antibody (8E417)
Anti-ASGR1 Antibody (8E417)
😃Good
Catalog No. TMAY-01152Alias HL-1, CLEC4H1, asialoglycoprotein receptor 1, ASGPR1, ASGPR
Anti-ASGR1 Antibody (8E417) is a Rabbit antibody targeting ASGR1. Anti-ASGR1 Antibody (8E417) can be used in WB,ELISA(Cap),IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $292 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA(Cap), IP Reactivity: Human |
| Alias | HL-1, CLEC4H1, asialoglycoprotein receptor 1, ASGPR1, ASGPR |
| Ig Type | Rabbit IgG |
| Clone | 8E417 |
| Reactivity | Human |
| Verified Activity | 1. Anti-ASGR1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:33 kDa. -Observed band size:43 kDa. 2. ASGR1 was immunoprecipitated using: -Lane A:0.5 mg HepG2 Whole Cell Lysate. -4 µL anti-ASGR1 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-ASGR1 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 33 kDa. -Observed band size:43 kDa |
| Application | WB,ELISA(Cap),IP |
| Recommended Dose | WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ASGPR1 (rh ASGPR1 ; TMPY-02702; P07306; Gln62-Leu291). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ASGPR1 protein (TMPY-02702) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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