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Anti-Coagulation factor IX/F9 Antibody (1B255) (Synonyms: coagulation factor IX)

Catalog No. TMAY-01880 Copy Product Info
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Anti-Coagulation factor IX/F9 Antibody (1B255) is a Rabbit antibody targeting Coagulation factor IX/F9. Anti-Coagulation factor IX/F9 Antibody (1B255) can be used in ELISA,IP,WB.

Anti-Coagulation factor IX/F9 Antibody (1B255)

Copy Product Info
🥰Excellent
Catalog No. TMAY-01880
Synonyms coagulation factor IX

Anti-Coagulation factor IX/F9 Antibody (1B255) is a Rabbit antibody targeting Coagulation factor IX/F9. Anti-Coagulation factor IX/F9 Antibody (1B255) can be used in ELISA,IP,WB.

Anti-Coagulation factor IX/F9 Antibody
(1B255)
Pack SizePriceUSA StockGlobal StockQuantity
20 μL$937-10 days7-10 days
100 μL$2937-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Coagulation factor IX/F9 Antibody (1B255) is a Rabbit antibody targeting Coagulation factor IX/F9. Anti-Coagulation factor IX/F9 Antibody (1B255) can be used in ELISA,IP,WB.
Synonymscoagulation factor IX
Ig Type
Rabbit IgG
Clone
1B255
Reactivity
Human
Specificity
Human Factor IX
Verified Activity
1. Anti-F9 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: A431 Whole Cell Lysate.
-Lane B: Jurkat Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:52 kDa.
-Observed band size:55 kDa.
2. F9 was immunoprecipitated using:
-Lane A:0.5 mg A431 Whole Cell Lysate.
-Lane B:0.5 mg Jurkat Whole Cell Lysate
-0.5 µL anti-F9 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
-Primary antibody:
-Anti-F9 rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size: 55 kDa.
-Observed band size:55 kDa
Application
Recommended Dose
WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IP: 0.1-0.5 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Coagulation Factor IX / FIX / F9 (rh Coagulation Factor IX / FIX / F9; TMPY-02215; AAB59620.1; Met 1-Thr 461).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCoagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human Coagulation Factor IX / FIX / F9 protein (TMPY-02215)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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