IGF1R/CD221 Protein, Human, Recombinant (His)

Catalog No. TMPY-00834

Synonyms: IGF-I R, IGFR, IGFIR, IGF1 Receptor, JTK13, insulin-like growth factor 1 receptor, CD221

The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. The IGF1R signaling pathway is activated in the mammalian nervous system from the early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signaling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumors and mediates proliferation and apoptosis protection. IGF signaling also influences hypoxia signaling, protease secretion, tumor cell motility, and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, IGF1R is now an attractive anti-cancer treatment target.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

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IGF1R/CD221 Protein, Human, Recombinant (His)

Pack Size	Availability	Price/USD
100 μg	In stock	$ 346.00
200 μg	5 days	$ 611.00
500 μg	5 days	$ 1,290.00

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COA DATASHEET SDS

Biological Description

Technical Params

Product Properties

References and Literature

Biological Information

1. Immobilized Anti-IGF-1R Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human IGF1R / CD221 Protein (His Tag) , the EC50 is 1.2-3.6 ng/mL(QC tested). 2.Captured IGF1R on NTA Chip can bind IGF1 with an affinity constant of 0.1317 µM as determined in a SPR assay (Routinely tested). 3.Loaded Anti-IGF-1R Antibody, human IgG1 on ProA Biosensor, can bind Recombinant Human IGF1R Protein, His Tag with an affinity constant of 0.808nM as determined in BLI assay (Routinely tested).

Description

Species	Human
Expression System	HEK293
Tag	His
Accession Number	P08069
Synonyms	IGF-I R, IGFR, IGFIR, IGF1 Receptor, JTK13, insulin-like growth factor 1 receptor, CD221
Construction	A DNA sequence encoding the extracellular domain (Met1-Asn932) of human IGF1R (NP_000866.1) was expressed with a C-terminal polyhistidine tag.
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	Approxiamtely 81 kDa

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two α and two β subunits, and among which, the α subunit is extracellular while the β subunit has an extracellular domain, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. The IGF1R signaling pathway is activated in the mammalian nervous system from the early developmental stages. Its major effect on developing neural cells is to promote their growth and survival. This pathway can integrate its action with signaling pathways of growth and morphogenetic factors that induce cell fate specification and selective expansion of specified neural cell subsets. Modulation of cell migration is another possible role that IGF1R activation may play in neurogenesis. In the mature brain, IGF-I binding sites have been found in different regions of the brain, and multiple reports confirmed a strong neuroprotective action of the IGF-IR against different pro-apoptotic insults. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells. IGF1R is frequently overexpressed by tumors and mediates proliferation and apoptosis protection. IGF signaling also influences hypoxia signaling, protease secretion, tumor cell motility, and adhesion, and thus can affect the propensity for invasion and metastasis. Therefore, IGF1R is now an attractive anti-cancer treatment target.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

References and Literature

1. Bhr C, et al. (2004) The insulin like growth factor-1 receptor (IGF-1R) as a drug target: novel approaches to cancer therapy. Growth Horm IGF Res. 14 (4): 287-95. 2. Riedemann J, et al. (2006) IGF1R signalling and its inhibition. Endocr Relat Cancer. 13 Suppl 1: 33-43. 3. Gualco E, et al. (2009) IGF-IR in neuroprotection and brain tumors. 4. Front Biosci. 14: 352-75.Annenkov A. (2009) The insulin-like growth factor (IGF) receptor type 1 (IGF1R) as an essential component of the signalling network regulating neurogenesis. Mol Neurobiol. 40 (3): 195-215.

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Keywords

IGF1R/CD221 Protein, Human, Recombinant (His) IGF-I R CD-221 IGFR IGFIR JTK-13 IGF1 Receptor JTK13 CD 221 JTK 13 insulin-like growth factor 1 receptor CD221 recombinant recombinant-proteins proteins protein