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Anti-CHRM3 Antibody (4D146)
😃Good
Catalog No. TMAH-00272
Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA,IHC,WB.
Anti-CHRM3 Antibody (4D146)
Anti-CHRM3 Antibody (4D146)
😃Good
Catalog No. TMAH-00272
Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA,IHC,WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $208 | 7-10 days | 7-10 days | |
| 100 μL | $348 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA,IHC,WB. |
| Ig Type | Rabbit IgG |
| Clone | 4D146 |
| Reactivity | Human |
| Verified Activity | 1. IHC image of TMAH-00272 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody 2. IHC image of TMAH-00272 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Muscarinic Acetylcholine Receptor M3 |
| Antigen Species | Human |
| Gene Name | CHRM3 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Neuroscience |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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