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Anti-CHRM3 Antibody (4D146)

Catalog No. TMAH-00272 Copy Product Info
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Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA,IHC,WB.

Anti-CHRM3 Antibody (4D146)

Copy Product Info
🥰Excellent
Catalog No. TMAH-00272

Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA,IHC,WB.

Anti-CHRM3 Antibody
(4D146)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2087-10 days7-10 days
100 μL$3487-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-CHRM3 Antibody (4D146) is an antibody targeting CHRM3. Anti-CHRM3 Antibody (4D146) can be used in ELISA,IHC,WB.
Ig Type
Rabbit IgG
Clone
4D146
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00272 diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
2. IHC image of TMAH-00272 diluted at 1:300 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Application
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundThe muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Muscarinic Acetylcholine Receptor M3
Antigen Species
Human
Gene Name
CHRM3
Gene ID
Uniprot ID
Biology Area
Neuroscience
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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