LCAT Protein, Human, Recombinant (His)

Name: LCAT Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-00055
Price: 67 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: lecithin-cholesterol acyltransferase) Copy Product Info

Synonyms: lecithin-cholesterol acyltransferase

Catalog No. TMPY-00055 Copy Product Info

LCAT Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48.2 kDa and the accession number is P04180.

Pack Size	Price	USA Stock	Global Stock
5 µg	$67	-	In Stock
10 µg	$107	-	In Stock
20 µg	$175	-	In Stock
50 µg	$343	-	In Stock
100 µg	$577	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human LCAT, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 13.0 ng/ml determined by ELISA.
Description	LCAT Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 48.2 kDa and the accession number is P04180.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P04180
Construction	Phe25-Glu440
Protein Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	lecithin-cholesterol acyltransferase
Research Background	Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.

Chemical Properties

Molecular Weight

48.2 kDa (Predicted); 65-70 kDa (Due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.