LCAT Protein, Human, Recombinant (His)

Lecithin: cholesterol acyltransferase (LCAT) is the sole enzyme that esterifies cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport. Lecithin cholesterol acyltransferase (LCAT) plays a pivotal role in HDL metabolism.LCAT is intimately involved in HDL maturation and is a key component of the reverse cholesterol transport (RCT) pathway which removes excess cholesterol molecules from the peripheral tissues to the liver for excretion. LCAT may also modify oxidative and inflammatory processes, as supported by an inverse relationship with HDL antioxidative functionality and a positive relationship with high-sensitivity C-reactive protein (hsCRP).

Pack Size	Availability	Price/USD	Quantity
100 μg	5 days	$ 498.00

Pack Size

Availability

Price/USD

Quantity

100 μg

5 days

$ 498.00

Description

Species	Human
Expression System	HEK293
Tag	His
Accession Number	A0A140VK24
Synonyms	lecithin-cholesterol acyltransferase
Construction	A DNA sequence encoding the human LCAT (NP_000220.1) (Met1-Glu440) was expressed with a polyhistidine tag at the C-terminus.
Protein Purity	> 85 % as determined by SDS-PAGE.
Molecular Weight	Approxiamtely 48.5 kDa

Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Lecithin: cholesterol acyltransferase (LCAT) is the sole enzyme that esterifies cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport. Lecithin cholesterol acyltransferase (LCAT) plays a pivotal role in HDL metabolism.LCAT is intimately involved in HDL maturation and is a key component of the reverse cholesterol transport (RCT) pathway which removes excess cholesterol molecules from the peripheral tissues to the liver for excretion. LCAT may also modify oxidative and inflammatory processes, as supported by an inverse relationship with HDL antioxidative functionality and a positive relationship with high-sensitivity C-reactive protein (hsCRP).

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LCAT Protein, Human, Recombinant (His)

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