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Anti-ABHD14B Antibody (2N426) is a Rabbit antibody targeting ABHD14B. Anti-ABHD14B Antibody (2N426) can be used in ELISA,ICC/IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μL | $269 | 7-10 days |
Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ICC/IF Reactivity: Human |
Alias | HEL-S-299, CIB, abhydrolase domain containing 14B |
Ig Type | Rabbit IgG |
Clone | 2N426 |
Reactivity | Human |
Verified Activity | Immunofluorescence staining of ABHD14B in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human ABHD14B monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to nucleus. |
Application | ELISA,ICC/IF |
Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
Antibody Type | Monoclonal |
Host Species | Rabbit |
Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (rh ABHD14B; TMPY-02978; Q96IU4-1; Met1-Gln210). |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human ABHD14B Protein (TMPY-02978) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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