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MICB Protein, Human, Recombinant (His & Avi)
(Synonyms: PERB11.2, MIC-B, MICB) Copy Product InfoSynonyms: PERB11.2, MIC-B, MICB
Catalog No. TMPK-00245 Copy Product Info
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. MICB Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 34.4 kDa and the accession number is Q29980-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $58 | 7-10 days | 7-10 days | |
| 10 μg | $92 | 7-10 days | 7-10 days | |
| 20 μg | $148 | 7-10 days | 7-10 days | |
| 50 μg | $289 | 7-10 days | 7-10 days | |
| 100 μg | $487 | 7-10 days | 7-10 days | |
| 200 μg | $883 | 7-10 days | 7-10 days | |
| 500 μg | $1,950 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Human MICB, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-MICB Antibody, hFc Tag with the EC50 of 42.4ng/ml determined by ELISA.
2. Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Human MICB, His Tag with an affinity constant of 83.31 nM as determined in SPR assay. |
| Description | MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. MICB Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 34.4 kDa and the accession number is Q29980-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q29980-1 |
| Construction | Ala23-Gly298 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | PERB11.2, MIC-B, MICB |
| Research Background |
| Molecular Weight | 34.4 kDa (predicted). Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: MICB Protein, Human, Recombinant (His & Avi) chemical structure | MICB Protein, Human, Recombinant (His & Avi) molecular weight
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