IL-21R Protein, Human, Recombinant (His)

Name: IL-21R Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01934
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: NILR, interleukin 21 receptor, CD360) Copy Product Info

Synonyms: NILR, interleukin 21 receptor, CD360

Catalog No. TMPY-01934 Copy Product Info

IL-21R Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.5 kDa and the accession number is Q9HBE5-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$32	-	In Stock
10 µg	$47	-	In Stock
20 µg	$74	-	In Stock
50 µg	$138	-	In Stock
100 µg	$228	-	In Stock
200 µg	$405	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:SDS-PAGE: 95.2%; SEC-HPLC: 90.5%

Appearance:Lyophilized powder

Color:White

Resource Download

COA User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	1. Measured by its ability to bind human IL-21 in a functional ELISA. 2.Measured by its ability to inhibit IL-21-induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 4-25 ng/mL in the presence of 2ng/mL of recombinant human IL-21.
Description	IL-21R Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.5 kDa and the accession number is Q9HBE5-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q9HBE5-1
Construction	A DNA sequence encoding the human IL21R (Q9HBE5-1) extracellular domain (Met 1-Pro 236) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Cys 20
Protein Purity	> 92 % as determined by SDS-PAGE. >90% as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	NILR, interleukin 21 receptor, CD360
Research Background	Interleukin-21 receptor, also known as IL-21 receptor, IL-21R, Novel interleukin receptor, IL21R, and NILR, is a single-pass type I membrane protein that belongs to the type I cytokine receptor family and Type 4 subfamily. Interleukin-21 (IL-21) belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL-21R) and the common cytokine receptor gamma chain ( gamma(C) ). The IL-21R is discovered as a novel member of the class-I-cytokine-receptor family and is selectively expressed in lymphoid tissues. IL-21R shows strong sequence homologies to the interleukin-4 receptor alpha chain gene (IL-4RA). The WSXWS motif of IL-21R appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif of IL-21R is required for JAK interaction and/or activation. The IL-21R is widely distributed on lymphohematopoietic cells and IL21 impacts some cell types, including CD8+ memory T cells, NK cells, and subsets of CD4 memory T cells. Increased IL21 production is characteristic of certain autoimmune diseases and is likely to contribute to autoantibody production as well as pathological features of autoimmune disease. The critical role of IL21 in promoting humoral immune responses makes it an important focus of potential therapeutic interventions in conditions characterized by the overproduction of pathogenic autoantibodies.

Chemical Properties

Molecular Weight

26.5 kDa (predicted); 42-46 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.