1. Immobilized Human IL-6 at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7 ng/ml determined by ELISA.
2. Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
3. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/mL (QC Test).

IL-6 Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P05231.

Human

E. coli

Interleukin-6,interleukin 6,IL-6,IFNB2,HSF,HGF,BSF2

Val30-Met212

> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.