1. Immobilized Human IL-6 at 0.5 μg/ml (100 μl/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 48.7 ng/ml determined by ELISA.
2. Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
3. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.1-0.5 ng/mL (QC Test).

IL-6 Protein, Human, Recombinant V2 is expressed in E. coli. The accession number is P05231.

Human

E. coli

Interleukin-6,interleukin 6,IL-6,IFNB2,HSF,HGF,BSF2

Val30-Met212

> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression.Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein.