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Anti-MYBBP1A Antibody (6F573) is an antibody targeting MYBBP1A. Anti-MYBBP1A Antibody (6F573) can be used in ELISA, IHC, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC, IF Reactivity: Human |
Alias | P160, MYBBP 1A, Myb-binding protein 1A |
Ig Type | Rabbit IgG |
Clone | 6F573 |
Reactivity | Human |
Verified Activity | 1. IHC image of TMAH-00791 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 2. Immunofluorescence staining of HepG2 Cells with TMAH-00791 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, IHC, IF |
Recommended Dose | IHC:1:50-1:200; IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter. Has a role in rRNA biogenesis together with PWP1. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human MYBBP1A |
Antigen Species | Human |
Gene ID | 10514 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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