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Anti-Aminoacylase 1 Antibody (5J317)

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Catalog No. TMAY-00961
Alias HEL-S-5, aminoacylase 1, ACY1D, ACY-1

Anti-Aminoacylase 1 Antibody (5J317) is a Mouse antibody targeting Aminoacylase 1. Anti-Aminoacylase 1 Antibody (5J317) can be used in WB,ELISA.

Anti-Aminoacylase 1 Antibody (5J317)

Anti-Aminoacylase 1 Antibody (5J317)

😃Good
Catalog No. TMAY-00961Alias HEL-S-5, aminoacylase 1, ACY1D, ACY-1
Anti-Aminoacylase 1 Antibody (5J317) is a Mouse antibody targeting Aminoacylase 1. Anti-Aminoacylase 1 Antibody (5J317) can be used in WB,ELISA.
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100 μL$198 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Aminoacylase 1 Antibody (5J317) is a Mouse antibody targeting Aminoacylase 1. Anti-Aminoacylase 1 Antibody (5J317) can be used in WB,ELISA.
AliasHEL-S-5, aminoacylase 1, ACY1D, ACY-1
Ig Type
Monoclonal Mouse IgG1
Clone
5J317
Reactivity
Human
Verified Activity
Anti-ACY1 mouse monoclonal antibody at 1:500 dilution.

-Lane A: K562 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:50 kDa.

-Observed band size:45 kDa
Application
WB,ELISA
Recommended Dose
WB: 1:500-1:1000; ELISA: 1:5000-1:10000
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACY1 (rh ACY1; TMPY-01058; NP_000657.1; Met 1-Ser 408). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundAminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human ACY1 protein (TMPY-01058)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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