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Anti-Aminoacylase 1 Antibody (5J317)
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Catalog No. TMAY-00961
Alias HEL-S-5, aminoacylase 1, ACY1D, ACY-1
Anti-Aminoacylase 1 Antibody (5J317) is a Mouse antibody targeting Aminoacylase 1. Anti-Aminoacylase 1 Antibody (5J317) can be used in WB,ELISA.
Anti-Aminoacylase 1 Antibody (5J317)
Anti-Aminoacylase 1 Antibody (5J317)
😃Good
Catalog No. TMAY-00961Alias HEL-S-5, aminoacylase 1, ACY1D, ACY-1
Anti-Aminoacylase 1 Antibody (5J317) is a Mouse antibody targeting Aminoacylase 1. Anti-Aminoacylase 1 Antibody (5J317) can be used in WB,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA Reactivity: Human |
| Alias | HEL-S-5, aminoacylase 1, ACY1D, ACY-1 |
| Ig Type | Mouse IgG1 |
| Clone | 5J317 |
| Reactivity | Human |
| Verified Activity | Anti-ACY1 mouse monoclonal antibody at 1:500 dilution. -Lane A: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:50 kDa. -Observed band size:45 kDa |
| Application | WB,ELISA |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ACY1 (rh ACY1; TMPY-01058; NP_000657.1; Met 1-Ser 408). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Aminoacylase 1 (ACY1), a metalloenzyme that removes amide-linked ACY1 groups from amino acids and may play a role in regulating responses to oxidative stress. Both the C-terminal fragment found in the two-hybrid screen and full-length ACY1 co-immunoprecipitate with SphK1. Though both C-terminal and full-length proteins slightly reduce SphK1 activity measured in vitro, the C-terminal fragment inhibits while full-length ACY1 potentiates the effects of SphK1 on proliferation and apoptosis. It suggested that ACY1 physically interacts with SphK1 and may influence its physiological functions. As a homodimeric zinc-binding enzyme, Aminoacylase 1 catalyzes the hydrolysis of N alpha-acylated amino acids. Deficiency of Aminoacylase 1 due to mutations in the Aminoacylase 1 (ACY1) gene follows an autosomal-recessive trait of inheritance and is characterized by accumulation of N-acetyl amino acids in the urine. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human ACY1 protein (TMPY-01058) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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