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Anti-Phospho-HSF1 (Ser362) Antibody (5H836)
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Catalog No. TMAH-00909
Alias p-HSF1 (Ser362), p-HSF1 (S326), Phospho-HSF1 (S326), HSTF1, HSF1 (p-Ser362), HSF1 (p-S326), heat shock transcription factor 1
Anti-Phospho-HSF1 (Ser362) Antibody (5H836) is an antibody targeting Phospho-HSF1 (Ser362). Anti-Phospho-HSF1 (Ser362) Antibody (5H836) can be used in ELISA, WB, ICC.
Anti-Phospho-HSF1 (Ser362) Antibody (5H836)
Anti-Phospho-HSF1 (Ser362) Antibody (5H836)
😃Good
Catalog No. TMAH-00909Alias p-HSF1 (Ser362), p-HSF1 (S326), Phospho-HSF1 (S326), HSTF1, HSF1 (p-Ser362), HSF1 (p-S326), heat shock transcription factor 1
Anti-Phospho-HSF1 (Ser362) Antibody (5H836) is an antibody targeting Phospho-HSF1 (Ser362). Anti-Phospho-HSF1 (Ser362) Antibody (5H836) can be used in ELISA, WB, ICC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, ICC Reactivity: Human |
| Alias | p-HSF1 (Ser362), p-HSF1 (S326), Phospho-HSF1 (S326), HSTF1, HSF1 (p-Ser362), HSF1 (p-S326), heat shock transcription factor 1 |
| Ig Type | Rabbit IgG |
| Clone | 5H836 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in Hela whole cell lysate -All lanes Phospho-HSF1 antibody at 0.8μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 82 KDa -Observed band size: 82 KDa 2. Immunocytochemistry analysis of TMAH-00909 diluted at 1:80 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Application | ELISA, WB, ICC |
| Recommended Dose | WB:1:500-1:5000; ICC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner. (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-HSF1 (S326) |
| Antigen Species | Human |
| Gene ID | 3297 |
| Uniprot ID | |
| Biology Area | Tags & Cell Markers |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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