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Anti-ECFP Antibody (5V334)
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Catalog No. TMAB-05421
Anti-ECFP Antibody (5V334) is a Mouse antibody targeting ECFP. Anti-ECFP Antibody (5V334) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-ECFP Antibody (5V334)
Anti-ECFP Antibody (5V334)
Copy Product Info😃Good
Catalog No. TMAB-05421
Anti-ECFP Antibody (5V334) is a Mouse antibody targeting ECFP. Anti-ECFP Antibody (5V334) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 100 μL | $185 | 7-10 days | 7-10 days | |
| 500 μL | $755 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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| Description | Anti-ECFP Antibody (5V334) is a Mouse antibody targeting ECFP. Anti-ECFP Antibody (5V334) can be used in ELISA, FCM, ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Ig Type | IgG |
| Clone | 5V334 |
| Reactivity | Species independent |
| Verified Activity | Sample: Lane 1: ECFP-Tagged fusion protein (full length) Overexpression E. Coli Lysate at 4 μg Primary: Anti-ECFP-Tag (TMAB-05421) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD |
| Application | |
| Recommended Dose | ELISA=1:1000-5000; FCM=1 μg/Test; ICC/IF=1:100-500; IF=1:200-1000; IHC-Fr=1:200-1000; IHC-P=1:200-1000; WB=1:1000-5000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Research Background | Cyan Fluorescent Protein (CFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. The fluorescence of EYFP was detected with an excitation filter of 485 nm and an emission filter of 545 nm, and that of ECFP was detected with filters of 420 and 486 nm (Perkin Elmer), respectively. In the present reporter assay, EYFP and ECFP were selected as a reporter protein and an internal control for normalization of transfection, respectively. |
| Immunogen | Recombinant Protein: ECFP |
| Protein Name | ECFP Tag |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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