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Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)
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Catalog No. TMAY-00008
Alias IGHG3
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) is a Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) can be used in ELISA.
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)
😃Good
Catalog No. TMAY-00008Alias IGHG3
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) is a Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) can be used in ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $68 | 7-10 days | |
| 100 μL | $233 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA Reactivity: Mouse |
| Alias | IGHG3 |
| Ig Type | Rabbit IgG |
| Clone | 7Z583 |
| Reactivity | Mouse |
| Verified Activity | 1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG3 Secondary Antibody reacts only to mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.1 μg/mL of Rabbit Anti-Mouse IgG3 Secondary Antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody. 2. Immunohistochemical analysis of PFA-fixed paraffin-embedded human liver tissue sections, with VEGF primary antibody (1/500) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/6400) was used as the secondary antibody. (Validation Experiment) 3. Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KRT7 primary antibody (1/200) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/3200) was used as the secondary antibody. (Validation Experiment) 4. Anti-ENO3 mouse monoclonal antibody at 1:1000 dilution. -Lane A: HepG2 Whole Cell Lysate. -Lane B: HeLa Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Rabbit Anti-Mouse IgG3/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:47 kDa. -Observed band size:47 kDa(Validation Experiment) |
| Application | ELISA |
| Recommended Dose | ELISA: 1:5000-1:10000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse IgG3. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | IGHG3 (Immunoglobulin Heavy Constant Gamma 3 (G3m Marker), also known as IgG3) is a Protein Coding gene. Ig gamma-3 chain C region is a protein that in humans is encoded by the IGHG3 gene. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Murine immunoglobulin G (IgG) plays an important role in mediating protective immune responses to malaria. Diseases associated with IGHG3 include Heavy Chain Disease and Gamma Heavy Chain Disease. Among its related pathways are IL4-mediated signaling events and the Creation of C4 and C2 activators. |
| Conjucates | Unconjugated |
| Others Formats | HRP |
| Antibody Types Available | 2 |
| Immunogen | Recombinant Protein: Mouse IgG3 Protein |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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