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Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)

😃Good
Catalog No. TMAY-00008
Alias IGHG3

Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) is a Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) can be used in ELISA.

Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)

Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583)

😃Good
Catalog No. TMAY-00008Alias IGHG3
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) is a Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) can be used in ELISA.
Pack SizePriceAvailabilityQuantity
20 μL$68 7-10 days
100 μL$233 7-10 days
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Product Introduction

Bioactivity
Description
Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) is a Rabbit antibody targeting IgG3. Rabbit Anti-Mouse IgG3 Secondary Antibody (7Z583) can be used in ELISA.
AliasIGHG3
Ig Type
Monoclonal Rabbit IgG
Clone
7Z583
Reactivity
Mouse
Verified Activity
1. ELISA of mouse immunoglobulins shows Rabbit Anti-Mouse IgG3 Secondary Antibody reacts only to mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM. The plate was coated with 0.1 μg/mL of different mouse immunoglobulins. 0.1 μg/mL of Rabbit Anti-Mouse IgG3 Secondary Antibody was used as the primary antibody. An HRP conjugated anti-Rabbit IgG as the secondary antibody.

2. Immunohistochemical analysis of PFA-fixed paraffin-embedded human liver tissue sections, with VEGF primary antibody (1/500) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/6400) was used as the secondary antibody. (Validation Experiment)

3. Immunohistochemical analysis of PFA-fixed paraffin-embedded human prostate tissue sections, with KRT7 primary antibody (1/200) for 1 hours at 37°C. An HRP-conjugated Rabbit Anti-Mouse IgG3 Secondary Antibody (1/3200) was used as the secondary antibody. (Validation Experiment)

4. Anti-ENO3 mouse monoclonal antibody at 1:1000 dilution.

-Lane A: HepG2 Whole Cell Lysate.

-Lane B: HeLa Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Rabbit Anti-Mouse IgG3/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size:47 kDa.

-Observed band size:47 kDa(Validation Experiment)
Application
ELISA
Recommended Dose
ELISA: 1:5000-1:10000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse IgG3.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundIGHG3 (Immunoglobulin Heavy Constant Gamma 3 (G3m Marker), also known as IgG3) is a Protein Coding gene. Ig gamma-3 chain C region is a protein that in humans is encoded by the IGHG3 gene. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Murine immunoglobulin G (IgG) plays an important role in mediating protective immune responses to malaria. Diseases associated with IGHG3 include Heavy Chain Disease and Gamma Heavy Chain Disease. Among its related pathways are IL4-mediated signaling events and the Creation of C4 and C2 activators.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
HRP
Antibody Types Available
2
Antigen Details
Immunogen
Recombinant Mouse IgG3 Protein
Antigen Species
Mouse
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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