Nectin-3 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05495

Nectin-3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.4 kDa and the accession number is Q9NQS3-1.

Nectin-3 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05495

Nectin-3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.4 kDa and the accession number is Q9NQS3-1.

Pack Size	Price	Availability	Quantity
100 μg	$600	7-10 days
1 mg	$3,910	7-10 days

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Product Information

Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag) at 2 μg/ml (100 μl/well) can bind Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag)is 30-140 ng/mL.
Description	Nectin-3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.4 kDa and the accession number is Q9NQS3-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q9NQS3-1
Synonyms	PVRR3,PVRL3,PRR3,PPR3,poliovirus receptor-related 3,nectin-3,FLJ90624,DKFZp566B0846,CDw113,CD113
Construction	A DNA sequence encoding the human NECTIN3 (NP_056295.1) (Met1-Asp400) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Gly 58
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	64.4 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in the reduction of cell movement and proliferation.