Nectin-3 Protein, Human, Recombinant (hFc)

Name: Nectin-3 Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05495
Rating: 4.5 (1 reviews)

(Synonyms: PVRR3, PVRL3, PRR3, PPR3, poliovirus receptor-related 3, nectin-3, FLJ90624, DKFZp566B0846, CDw113, CD113) Copy Product Info

Synonyms: PVRR3, PVRL3, PRR3, PPR3, poliovirus receptor-related 3, nectin-3, FLJ90624, DKFZp566B0846, CDw113, CD113

Catalog No. TMPY-05495 Copy Product Info

Nectin-3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.4 kDa and the accession number is Q9NQS3-1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$68	7-10 days	7-10 days
10 µg	$108	7-10 days	7-10 days
20 µg	$178	7-10 days	7-10 days
50 µg	$359	7-10 days	7-10 days
100 µg	$696	7-10 days	7-10 days
200 µg	$1,060	7-10 days	7-10 days
500 µg	$2,230	7-10 days	7-10 days
1 mg	$4,530	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized PVRL1/NECTIN1 Protein, Human, Recombinant (His Tag) at 2 μg/ml (100 μl/well) can bind Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of Nectin 3 Protein, Human, Recombinant (ECD, hFc Tag)is 30-140 ng/mL.
Description	Nectin-3 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 64.4 kDa and the accession number is Q9NQS3-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc (IgG1)
Accession Number	Q9NQS3
Construction	Gly58-Asp400
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	PVRR3, PVRL3, PRR3, PPR3, poliovirus receptor-related 3, nectin-3, FLJ90624, DKFZp566B0846, CDw113, CD113
Research Background	Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in the reduction of cell movement and proliferation.

Chemical Properties

Molecular Weight

63.63 kDa (predicted); 80-110 kDa (due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.