CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-01990 Copy Product Info

CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.5 kDa and the accession number is Q8SPW3.

CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

Catalog No. TMPY-01990

Copy Product Info

CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated

Pack Size	Price	USA Stock	Global Stock
5 μg	$109	7-10 days	7-10 days
10 μg	$178	7-10 days	7-10 days
20 μg	$296	-	In Stock
50 μg	$478	7-10 days	7-10 days
100 μg	$690	7-10 days	7-10 days
200 μg	$1,180	7-10 days	7-10 days
500 μg	$2,380	7-10 days	7-10 days

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For In stock only · Estimated delivery:USA Stock (1-2 days) Global Stock (5-7 days)

For research use only—not for human use. No sales to individuals. Use as intended only.

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COA User Guide of Recombinant Proteins

Product Information

Biological Activity	Immobilized Cynomolgus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated at 2 μg/mL (100 μL/well) can bind Human IgG2 Protein (257 Ser/Ala) , the EC50 is 4-36 ng/mL.
Description	CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.5 kDa and the accession number is Q8SPW3.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	His, Avi
Accession Number	Q8SPW3
Synonyms	Fc fragment of IgG, low affinity IIb, receptor (CD32)
Construction	A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 45
Protein Purity	> 97 % as determined by SDS-PAGE
Molecular Weight	23.5 kDa (predicted); 34 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.