Anti-BID Antibody (1W750)

Name: Anti-BID Antibody (1W750)
Brand: TargetMol
SKU: TMAY-00871
Rating: 4.5 (1 reviews)

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Catalog No. TMAY-00871

Alias BH3 interacting domain death agonist, AU022477, AI875481, 2700049M22Rik

Anti-BID Antibody (1W750) is a Rabbit antibody targeting BID. Anti-BID Antibody (1W750) can be used in ELISA,ICC/IF.

Anti-BID Antibody (1W750)

😃Good

Catalog No. TMAY-00871Alias BH3 interacting domain death agonist, AU022477, AI875481, 2700049M22Rik

Anti-BID Antibody (1W750) is a Rabbit antibody targeting BID. Anti-BID Antibody (1W750) can be used in ELISA,ICC/IF.

Pack Size	Price	Availability	Quantity
100 μL	$270	7-10 days

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Instruction Manual of Detection Antibody

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Product Introduction

Bioactivity

Description	Antibody Type: Rabbit Monoclonal Application: ELISA, ICC/IF Reactivity: Human
Alias	BH3 interacting domain death agonist, AU022477, AI875481, 2700049M22Rik
Ig Type	Rabbit IgG
Clone	1W750
Reactivity	Human
Verified Activity	Immunofluorescence staining of Human BID in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human BID monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Application	ELISA,ICC/IF
Recommended Dose	ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100
Antibody Type	Monoclonal
Host Species	Rabbit
Construction	This antibody was obtained from a rabbit immunized with purified, recombinant Human BID (rh BID; TMPY-02027; P55957-1; Met1-Asp195).
Purification	Protein A
Appearance	Liquid
Formulation	0.2 μm filtered solution in PBS
Research Background	The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Related Conjugates and Formulations

Conjucates

Unconjugated

Antigen Details

Immunogen	Recombinant Protein: Human BID protein (TMPY-02027)
Antigen Species	Human

Chemical Properties

Stability & Storage

Stability & Storage	Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
Transport	Shipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc