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SARS-COV-2 Spike S1 Protein (D614G, His & Avi)
(Synonyms: Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1) Copy Product InfoSynonyms: Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1
Catalog No. TMPK-00436 Copy Product Info
The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $61 | - | In Stock | |
| 10 μg | $96 | - | In Stock | |
| 20 μg | $156 | - | In Stock | |
| 50 μg | $297 | - | In Stock | |
| 100 μg | $511 | - | In Stock | |
| 200 μg | $925 | 7-10 days | 7-10 days | |
| 500 μg | $2,030 | - | In Stock |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.7ng/ml determined by ELISA. |
| Description | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Species | SARS-CoV-2 |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | A0A6G7K2L4 |
| Construction | Gln14-Arg683(D614G) |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Spike,S1 protein, Spike protein S1, S1 protein, S glycoprotein Subunit1 |
| Research Background | The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct. |
| Molecular Weight | 77.9 kDa (predicted). Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
| Storage | Store at low temperature Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: SARS-COV-2 Spike S1 Protein (D614G, His & Avi) chemical structure | SARS-COV-2 Spike S1 Protein (D614G, His & Avi) molecular weight
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