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Anti-AKR1C3 Antibody (4G958)
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Catalog No. TMAH-00046
Alias Dihydrodiol Dehydrogenase, Chlordecone Reductase Homolog HAKRb, Aldo-Keto Reductase Family 1 Member C3, 3-α-Hydroxysteroid Dehydrogenase Type 2, 3-α-HSD Type II Brain, 3-α-HSD Type 2, 3-Alpha-Hydroxysteroid Dehydrogenase Type 2, 3-Alpha-HSD Type II Brain, 3-Alpha-HSD Type 2, 17-β-Hydroxysteroid Dehydrogenase Type 5, 17-β-HSD 5, 17-Beta-Hydroxysteroid Dehydrogenase Type 5, 17-Beta-HSD 5
Anti-AKR1C3 Antibody (4G958) is an antibody targeting AKR1C3. Anti-AKR1C3 Antibody (4G958) can be used in ELISA, IF, FC.
Anti-AKR1C3 Antibody (4G958)
Anti-AKR1C3 Antibody (4G958)
😃Good
Catalog No. TMAH-00046Alias Dihydrodiol Dehydrogenase, Chlordecone Reductase Homolog HAKRb, Aldo-Keto Reductase Family 1 Member C3, 3-α-Hydroxysteroid Dehydrogenase Type 2, 3-α-HSD Type II Brain, 3-α-HSD Type 2, 3-Alpha-Hydroxysteroid Dehydrogenase Type 2, 3-Alpha-HSD Type II Brain, 3-Alpha-HSD Type 2, 17-β-Hydroxysteroid Dehydrogenase Type 5, 17-β-HSD 5, 17-Beta-Hydroxysteroid Dehydrogenase Type 5, 17-Beta-HSD 5
Anti-AKR1C3 Antibody (4G958) is an antibody targeting AKR1C3. Anti-AKR1C3 Antibody (4G958) can be used in ELISA, IF, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Chemical Properties
| Description | Anti-AKR1C3 Antibody (4G958) is an antibody targeting AKR1C3. Anti-AKR1C3 Antibody (4G958) can be used in ELISA, IF, FC. |
| Alias | Dihydrodiol Dehydrogenase, Chlordecone Reductase Homolog HAKRb, Aldo-Keto Reductase Family 1 Member C3, 3-α-Hydroxysteroid Dehydrogenase Type 2, 3-α-HSD Type II Brain, 3-α-HSD Type 2, 3-Alpha-Hydroxysteroid Dehydrogenase Type 2, 3-Alpha-HSD Type II Brain, 3-Alpha-HSD Type 2, 17-β-Hydroxysteroid Dehydrogenase Type 5, 17-β-HSD 5, 17-Beta-Hydroxysteroid Dehydrogenase Type 5, 17-Beta-HSD 5 |
| Ig Type | Rabbit IgG |
| Clone | 4G958 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Hela Cells with TMAH-00046 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). 2. Overlay histogram showing A549 cells stained with TMAH-00046 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, IF, FC |
| Recommended Dose | IF:1:20-1:200; FC:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 17-ketosteroid reductase and has the highest cata... |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human AKR1C3 |
| Antigen Species | Human |
| Gene ID | 8644 |
| Uniprot ID | |
| Biology Area | Neuroscience, Cancer, Metabolism, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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