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EFEMP1 Protein, Human, Recombinant (His)

(Synonyms: S1-5, MTLV, MLVT, MGC111353, Fibulin 3, fibrillin-like, FIBL-3, FBNLFLJ35535, FBNL, FBLN3, EFEMP1, DHRD) Copy Product Info

Synonyms: S1-5, MTLV, MLVT, MGC111353, Fibulin 3, fibrillin-like, FIBL-3, FBNLFLJ35535, FBNL, FBLN3, EFEMP1, DHRD

Catalog No. TMPK-00946 Copy Product Info
Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. EFEMP1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 53.9 kDa and the accession number is Q12805-1.
EFEMP1 Protein, Human, Recombinant (His)
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Pack SizePriceUSA StockGlobal StockQuantity
5 µg$58-In Stock
10 µg$92-In Stock
20 µg$148-In Stock
50 µg$289-In Stock
100 µg$487-In Stock
200 µg$8837-10 days7-10 days
500 µg$1,9507-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human EFEMP1, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EFEMP1 Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
Description
Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. EFEMP1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 53.9 kDa and the accession number is Q12805-1.
Species
Human
Expression System
HEK293 Cells
TagN-His
Accession NumberQ12805-1
ConstructionGln18-Phe493
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsS1-5, MTLV, MLVT, MGC111353, Fibulin 3, fibrillin-like, FIBL-3, FBNLFLJ35535, FBNL, FBLN3, EFEMP1, DHRD
Research Background
Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion.
Chemical Properties
Molecular Weight53.9 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: EFEMP1 Protein, Human, Recombinant (His) chemical structure | EFEMP1 Protein, Human, Recombinant (His) molecular weight