Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. EFEMP1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 53.9 kDa and the accession number is Q12805-1.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 487.00 | |
500 μg | 5 days | $ 1,950.00 | |
1 mg | 5 days | $ 3,250.00 |
Description | Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. EFEMP1 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 53.9 kDa and the accession number is Q12805-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | N-His |
Accession Number | Q12805-1 |
Synonyms | MLVT, S1-5, Fibulin 3, FBNL, fibrillin-like, MGC111353, FBLN3, FBNLFLJ35535, MTLV, DHRD, EFEMP1, FIBL-3 |
Construction | Gln18-Phe493 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 53.9 kDa (predicted). Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping |
In general, Lyophilized powders are shipping with blue ice. |
Research Background | Transversalis fascia EFEMP1, TIMP3 and ELN expressions were decreased, but MMP9 expression was increased in IH patients compared with controls. In IH patients, EFEMP1 was not correlated with TIMP3, but positively correlated with ELN and negatively correlated with MMP9; TIMP3 negatively correlated with MMP9, but positively correlated with ELN.In addition, EFEMP1 suppressed L929 cell migration and invasion. |
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EFEMP1 Protein, Human, Recombinant (His) MLVT FBLN 3 S1-5 MGC 111353 Fibulin 3 FBNL fibrillin-like MGC-111353 FIBL 3 MGC111353 FBNLFLJ-35535 FBLN3 EFEMP 1 FIBL3 FBLN-3 FBNLFLJ35535 MTLV FBNLFLJ 35535 EFEMP-1 DHRD EFEMP1 FIBL-3 recombinant recombinant-proteins proteins protein