Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-IkB alpha/NFKBIA Antibody (4A483)

😃Good
Catalog No. TMAY-02206
Alias nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α

Anti-IkB alpha/NFKBIA Antibody (4A483) is a Rabbit antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (4A483) can be used in WB,ELISA,IP.

Anti-IkB alpha/NFKBIA Antibody (4A483)

Anti-IkB alpha/NFKBIA Antibody (4A483)

😃Good
Catalog No. TMAY-02206Alias nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-IkB alpha/NFKBIA Antibody (4A483) is a Rabbit antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (4A483) can be used in WB,ELISA,IP.
Pack SizePriceAvailabilityQuantity
100 μL255 € 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Anti-IkB alpha/NFKBIA Antibody (4A483) is a Rabbit antibody targeting IkB alpha/NFKBIA. Anti-IkB alpha/NFKBIA Antibody (4A483) can be used in WB,ELISA,IP.
Aliasnuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Ig Type
Monoclonal Rabbit IgG
Clone
4A483
Reactivity
Human
Verified Activity
1. Anti-NFKBIA rabbit polyclonal antibody at 1:500 dilution.

-Lane A: HepG2 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:37 kDa.

-Observed band size:38 kDa.

2. NFKBIA was immunoprecipitated using:

-Lane A:0.5 mg HepG2 Whole Cell Lysate.

-Lane B:0.5 mg 293T Whole Cell Lysate.

-4 µL anti-NFKBIA rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

-Primary antibody:

-Anti-NFKBIA rabbit monoclonal antibody, at 1:100 dilution.

-Secondary antibody:

-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

-Developed using the ECL technique.

-Performed under reducing conditions.

-Predicted band size: 37 kDa.

-Observed band size:38 kDa
Application
WB,ELISA,IP
Recommended Dose
WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human IkB alpha / NFKBIA (rh IkB alpha / NFKBIA; TMPY-01710; Phe2-Leu317; NP_065390.1) .
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundNuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IkB alpha, NFKBIA, or IKBA), is a member of the NF-kappa-B inhibitor family that function to inhibit the NF-kB transcription factor. NFKBIA inhibits NF-kB by masking the nuclear localization signals (NLS) of NF-kB proteins and keeping them sequestered in an inactive state in the cytoplasm. Also, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-kB's proper functioning. Signal-induced degradation of I kappa B alpha exposes the nuclear localization signal of NF-kappa B, thus allowing it to translocate into the nucleus and activate transcription from responsive genes. An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription. As part of this post-induction repression, the nuclear export signal on I kappa B alpha mediates the transport of NF-kappa B-I kappa B alpha complexes from the nucleus to the cytoplasm. Deletion of NFKBIA has an effect that is similar to the effect of EGFR amplification in the pathogenesis of glioblastoma and is associated with comparatively short survival. Polymorphisms in NFKBIA may be important in pre-disposition to and outcome after treatment, of multiple myeloma (MM). The NFKBIA gene product, IkappaBalpha, binds to NF-kappaB preventing its activation and is important in mediating resistance to apoptosis in B-cell lymphoproliferative diseases.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human IkB alpha / NFKBIA Protein (TMPY-01710)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-IkB alpha/NFKBIA Antibody (4A483) | purchase Anti-IkB alpha/NFKBIA Antibody (4A483) | Anti-IkB alpha/NFKBIA Antibody (4A483) cost | order Anti-IkB alpha/NFKBIA Antibody (4A483)