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Anti-CD25/IL2R alpha Antibody (1W266) is an antibody targeting CD25/IL2R alpha. Anti-CD25/IL2R alpha Antibody (1W266) can be used in ELISA, IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $208 | 7-10 days | |
100 μL | $348 | 7-10 days |
Description | Antibody Type: Recombinant Monoclonal Application: ELISA, IF Reactivity: Human |
Alias | TCGFR, p55, interleukin 2 receptor, α, interleukin 2 receptor, alpha, IL-2RA, IL2R, IDDM10, CD25/IL2R α, CD25 |
Ig Type | Human IgG1 |
Clone | 1W266 |
Reactivity | Human |
Verified Activity | 1. The Binding Activity of Human IL2RA with Anti-IL2RA Recombinant Antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 μg/mL can bind Anti-IL2RA recombinant antibody, the EC50 is 2.463-3.353 ng/mL. 2. Immunofluorescence staining of Hela cell with TMAH-00622 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L). 3. Immunofluorescence staining of HEPG2 cell with TMAH-00622 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-human IgG(H+L). |
Application | ELISA, IF |
Recommended Dose | IF:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Membrane; Single-pass type I membrane protein. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human IL2RA Protein |
Antigen Species | Human |
Gene ID | 3559 |
Uniprot ID | |
Biology Area | Immunology |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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