Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human

IgG2b

7P299

Human

1. Western Blot
-Positive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMA
-All lanes: TNFRSF17 antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 40, 15 KDa
-Observed band size: 40, 15 KDa
-Exposure time:10min
2. Immunofluorescence staining of 293F cells transfected with TNFRSF17 with TMAH-01190 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
3. Overlay histogram showing 293F cells transfected with TNFRSF17 stained with TMAH-01190 (red line) at 1:100. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC

Monoclonal

Unconjugated

Recombinant Protein: Human TNFRSF17 Protein (1-54AA)

Human

608

Immunology