DKK1 Protein, Human, Recombinant (hFc)

Name: DKK1 Protein, Human, Recombinant (hFc)
Brand: TargetMol
SKU: TMPY-05479
Rating: 4.5 (1 reviews)

(Synonyms: SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1) Copy Product Info

Synonyms: SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1

Catalog No. TMPY-05479 Copy Product Info

DKK1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 52.5 kDa and the accession number is I1W660.

Pack Size	Price	USA Stock	Global Stock
5 µg	$55	7-10 days	7-10 days
10 µg	$87	7-10 days	7-10 days
20 µg	$142	7-10 days	7-10 days
50 µg	$277	7-10 days	7-10 days
100 µg	$535	7-10 days	7-10 days
200 µg	$815	7-10 days	7-10 days
500 µg	$1,680	7-10 days	7-10 days
1 mg	$3,480	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Human DKK1, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 1.69 μg/ml determined by ELISA.
Description	DKK1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 52.5 kDa and the accession number is I1W660.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	O94907
Construction	Thr32-His266
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	SK, DKK-1, dickkopf WNT signaling pathway inhibitor 1
Research Background	Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Chemical Properties

Molecular Weight

52.5 kDa (predicted); 65-80 kDa (reducing conditions due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.