Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
100 μg | 5 days | $ 581.00 | |
500 μg | 5 days | $ 2,330.00 | |
1 mg | 5 days | $ 3,880.00 |
Description | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. |
Species | Human |
Expression System | HEK293 |
Tag | C-His-Avi |
Accession Number | P78324 variant 5 |
Synonyms | MFR, PTPNS1, P84, BIT, MYD-1, MYD1, SHPS-1, SIRPA, SIRP α, SIRP α V5, SHPS1, SIRP alpha, CD172a |
Construction | Recombinant Human SIRP alpha V5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Arg370(S42F).[Accession |P78324 variant 5] |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
Endotoxin | Less than 1EU per μg by the LAL method. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability & Storage |
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Shipping |
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Research Background | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. |
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Please read the User Guide of Recombinant Proteins for more specific information.
SIRP alpha V5 Protein, Human, Recombinant (His & Avi) MFR PTPNS1 P84 BIT SHPS 1 MYD-1 PTPNS-1 PTPNS 1 P-84 MYD1 SHPS-1 SIRPA SIRP α SIRP α V5 P 84 MYD 1 SHPS1 SIRP alpha CD172a recombinant recombinant-proteins proteins protein