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SIRP alpha V5 Protein, Human, Recombinant (His & Avi)
(Synonyms: SIRPA, SIRP α V5, SIRP α, SIRP alpha, SHPS-1, SHPS1, PTPNS1, P84, MYD-1, MYD1, MFR, CD172a, BIT) Copy Product InfoSynonyms: SIRPA, SIRP α V5, SIRP α, SIRP alpha, SHPS-1, SHPS1, PTPNS1, P84, MYD-1, MYD1, MFR, CD172a, BIT
Catalog No. TMPK-01357 Copy Product Info
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $68 | 7-10 days | 7-10 days | |
| 10 μg | $108 | 7-10 days | 7-10 days | |
| 20 μg | $177 | 7-10 days | 7-10 days | |
| 50 μg | $346 | 7-10 days | 7-10 days | |
| 100 μg | $581 | 7-10 days | 7-10 days | |
| 200 μg | $1,050 | 7-10 days | 7-10 days | |
| 500 μg | $2,330 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human SIRP alpha V5, His Tag at 1μg/ml (100μl/Well).Dose response curve for Human CD47, hFc Tag with the EC50 of 39.1ng/ml determined by ELISA. |
| Description | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P78324 variant 5 |
| Construction | Glu31-Arg370(S42F) |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | SIRPA, SIRP α V5, SIRP α, SIRP alpha, SHPS-1, SHPS1, PTPNS1, P84, MYD-1, MYD1, MFR, CD172a, BIT |
| Research Background |
| Molecular Weight | 40 kDa (predicted). Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: SIRP alpha V5 Protein, Human, Recombinant (His & Avi) chemical structure | SIRP alpha V5 Protein, Human, Recombinant (His & Avi) molecular weight
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