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Anti-HSF1 Antibody (7F827)

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Catalog No. TMAY-02296
Alias HSTF1, heat shock transcription factor 1

Anti-HSF1 Antibody (7F827) is a Rabbit antibody targeting HSF1. Anti-HSF1 Antibody (7F827) can be used in ELISA,IHC-P,ICC/IF.

Anti-HSF1 Antibody (7F827)

Anti-HSF1 Antibody (7F827)

😃Good
Catalog No. TMAY-02296Alias HSTF1, heat shock transcription factor 1
Anti-HSF1 Antibody (7F827) is a Rabbit antibody targeting HSF1. Anti-HSF1 Antibody (7F827) can be used in ELISA,IHC-P,ICC/IF.
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100 μL$268 7-10 days
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Product Introduction

Bioactivity
Description
Anti-HSF1 Antibody (7F827) is a Rabbit antibody targeting HSF1. Anti-HSF1 Antibody (7F827) can be used in ELISA,IHC-P,ICC/IF.
AliasHSTF1, heat shock transcription factor 1
Ig Type
Monoclonal Rabbit IgG
Clone
7F827
Reactivity
Human
Verified Activity
1. Immunochemical staining of human HSF1 in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

2. Immunochemical staining of human HSF1 in human placenta with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).

3. Immunofluorescence staining of Human HSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSF1 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red).
Application
ELISA,IHC-P,ICC/IF
Recommended Dose
ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human HSF1 (rh HSF1; TMPY-02072; Q00613-1; Asp2-Ser529).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundHeat shock factor protein 1, also known as heat shock transcription factor 1, HSF1, and HSTF1, is a cytoplasm and nucleus protein that belongs to the HSF family. HSF1 is the major transcription factor of HSPs (heat shock proteins) in response to various stresses. Wild type HSF1 (heat shock transcriptional factor 1) is normally inactive. HSF1 / HSTF1 is a DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF1 / HSTF1 protects cells and organisms against various types of stress, either by triggering a complex response that promotes cell survival or by triggering cell death when stress-induced alterations cannot be rescued. HSF1 / HSTF1 is the key protein in regulating the stress response. It can be activated under heat, oxidative, or other stress conditions. Dominant-positive and dominant-negative HSF1 are two types of HSF1 mutants. Both of them gain DNA binding activity in the absence of stress. Also, dominant-positive HSF1 acquires transcriptional activity, which dominant-negative HSF1 does not acquire. HSF1 / HSTF1 was also reported to contribute to cell resistance against genotoxic stress, such as that caused by doxorubicin, an anticancer drug in common clinical use.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human HSF1 protein (TMPY-02072)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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