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Anti-Mono-methyl-Histone H4 (K16) Antibody (6M547) is an antibody targeting Mono-methyl-Histone H4 (K16). Anti-Mono-methyl-Histone H4 (K16) Antibody (6M547) can be used in ELISA, IF.
Description | Anti-Mono-methyl-Histone H4 (K16) Antibody (6M547) is an antibody targeting Mono-methyl-Histone H4 (K16). Anti-Mono-methyl-Histone H4 (K16) Antibody (6M547) can be used in ELISA, IF. |
Ig Type | Rabbit IgG |
Clone | 6M547 |
Reactivity | Human, Rat |
Verified Activity | Immunofluorescence staining of Hela cells with TMAH-00777 at 1:50,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
Application | ELISA, IF |
Recommended Dose | IF:1:30-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Nucleus. Chromosome. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
Conjucates | Unconjugated |
Immunogen | A synthesized peptide |
Antigen Species | Human |
Gene ID | 121504 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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