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Anti-AIF Antibody (2K106)
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Catalog No. TMAH-00044
Alias Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Anti-AIF Antibody (2K106) is an antibody targeting AIF. Anti-AIF Antibody (2K106) can be used in ELISA, WB, IHC.
Anti-AIF Antibody (2K106)
Anti-AIF Antibody (2K106)
😃Good
Catalog No. TMAH-00044Alias Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF
Anti-AIF Antibody (2K106) is an antibody targeting AIF. Anti-AIF Antibody (2K106) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human, Rat |
| Alias | Programmed Cell Death Protein 8, PDCD8, Apoptosis-Inducing Factor 1 Mitochondrial, AIFM1, AIF |
| Ig Type | Rabbit IgG |
| Clone | 2K106 |
| Reactivity | Human, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Rat liver tissue, Rat kidney tissue -All lanes: AIFM1 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 67, 36, 29, 27 kDa -Observed band size: 67 kDa 2. IHC image of TMAH-00044 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00044 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region.; [Isoform 3]: Mitochondrion intermembrane space. Mitochondrion inner membrane.; [Isoform 4]: Mitochondrion. Cytoplasm, cytosol.; [Isoform 5]: Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import. Has NADH oxidoreductase activity. Does not induce nuclear apoptosis. Pro-apoptotic isoform. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human AIF |
| Antigen Species | Human |
| Gene ID | 9131 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Cell biology, Metabolism |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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