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LIFR Protein, Rat, Recombinant (His)
(Synonyms: leukemia inhibitory factor receptor α, leukemia inhibitory factor receptor alpha) Copy Product InfoSynonyms: leukemia inhibitory factor receptor α, leukemia inhibitory factor receptor alpha
Catalog No. TMPY-03306 Copy Product Info
LIFR Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 90 kDa and the accession number is G3V7K2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $68 | 7-10 days | 7-10 days | |
| 10 μg | $108 | 7-10 days | 7-10 days | |
| 20 μg | $178 | 7-10 days | 7-10 days | |
| 50 μg | $359 | 7-10 days | 7-10 days | |
| 100 μg | $696 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1.Measured by its binding ability in a functional ELISA. Immobilized Mouse LIF hFc at 2 μg/ml (100 μl/well) can bind Rat LIFR His , the EC50 of Rat LIFR His is 300-700 ng/mL.
2. Measured by its ability to inhibit the recombinant human LIF mediated inhibition in the M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 8-40 ng/mL in the presence of 2 ng/mL recombinant human LIF. |
| Description | LIFR Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 90 kDa and the accession number is G3V7K2. |
| Species | Rat |
| Expression System | HEK293 Cells |
| Tag | C-His |
| Accession Number | G3V7K2 |
| Construction | A DNA sequence encoding the rat LIFR (G3V7K2) (Met1-Ser829) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 44 |
| Protein Purity | > 95 % as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | leukemia inhibitory factor receptor α, leukemia inhibitory factor receptor alpha |
| Research Background |
| Molecular Weight | 90 kDa (predicted); 116 kDa (reducing condition, due to glycosylation) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Dose Conversion
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Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: LIFR Protein, Rat, Recombinant (His) chemical structure | LIFR Protein, Rat, Recombinant (His) molecular weight
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