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Anti-Ribonuclease A Antibody (5T931) is a Mouse antibody targeting Ribonuclease A. Anti-Ribonuclease A Antibody (5T931) can be used in ELISA,ELISA(Cap).
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $68 | 7-10 days | |
100 μL | $199 | 7-10 days |
Description | Anti-Ribonuclease A Antibody (5T931) is a Mouse antibody targeting Ribonuclease A. Anti-Ribonuclease A Antibody (5T931) can be used in ELISA,ELISA(Cap). |
Alias | RNS1, ribonuclease, RNase A family, 1 (pancreatic), RIB1, RAC1 |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 5T931 |
Reactivity | Human |
Application | ELISA,ELISA(Cap) |
Recommended Dose | ELISA: 1:1000-1:2000; ELISA(Cap): 1:250-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RNase A / Ribonuclease A / RNASE1 (rh RNase A / Ribonuclease A / RNASE1; TMPY-03180; P07998; Met1-Thr156). The IgG fraction of the cell culture supernatant was purified by Protein A affinity. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in 20 mM MES, 150 mM NaCl, 10% Trehalose, 0.02% Tween 80, pH 6.0 |
Research Background | RNase A, also known as ribonuclease A and RNASE1, belongs to ribonuclease A superfamily. It is a pancreatic-type of secretory ribonuclease. RNase A is a basic protein and its many positive charges are consistent with its binding to RNA (a poly-anion). More generally, RNase A is unusually polar or, rather, unusually lacking in hydrophobic groups, especially aliphatic ones. As an endonuclease, RNase A cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. RNase A is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. |
Conjucates | Unconjugated |
Immunogen | Recombinant Human RNase A / Ribonuclease A / RNASE1 protein (TMPY-03180) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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