Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, IF

Reactivity: Human

Rabbit IgG

2P332

Human

1. IHC image of TMAH-01245 diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
2. Immunofluorescence staining of hela cell with TMAH-01245 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
3. Immunofluorescence staining of MCF7 cell with TMAH-01245 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELISA, IHC, IF

IHC:1:50-1:200; IF:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human VEGFA Protein

Human

7422

Cancer, Cardiovascular, Developmental biology, Metabolism, Signal transduction