Immobilized Human IGFBP-3, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA (QC Test).
Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).

IGFBP-3 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is AAH18962.

Human

HEK293 Cells

insulin-like growth factor binding protein 3,IGFBP3,IBP3,BP-53

Gly28-Lys291

> 95% as determined by Tris-Bis PAGE

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.