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Anti-Vitamin D Receptor/VDR Antibody (5I578)
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Catalog No. TMAH-01244
Alias vitamin D (1,25- dihydroxyvitamin D3) receptor, PPP1R163, NR1I1
Anti-Vitamin D Receptor/VDR Antibody (5I578) is an antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Antibody (5I578) can be used in ELISA, WB, IHC.
Anti-Vitamin D Receptor/VDR Antibody (5I578)
Anti-Vitamin D Receptor/VDR Antibody (5I578)
😃Good
Catalog No. TMAH-01244Alias vitamin D (1,25- dihydroxyvitamin D3) receptor, PPP1R163, NR1I1
Anti-Vitamin D Receptor/VDR Antibody (5I578) is an antibody targeting Vitamin D Receptor/VDR. Anti-Vitamin D Receptor/VDR Antibody (5I578) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | vitamin D (1,25- dihydroxyvitamin D3) receptor, PPP1R163, NR1I1 |
| Ig Type | Rabbit IgG |
| Clone | 5I578 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: MCF-7 whole cell lysate, THP-1 whole cell lysate, HL-60 whole cell lysate -All lanes: Vitamin D antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 49, 54 kDa -Observed band size: 49 kDa 2. IHC image of TMAH-01244 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Plays a central role in calcium homeostasis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Vitamin D Receptor |
| Antigen Species | Human |
| Gene ID | 7421 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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