Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC

Reactivity: Human

IgG2b

6D874

Human

1. Western Blot
-Positive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMA
-All lanes: TNFRSF17 antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 40, 15 KDa
-Observed band size: 40, 15 KDa
-Exposure time:10min
2. IHC image of TMAH-01189 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. IHC image of TMAH-01189 diluted at 1:100 and staining in paraffin-embedded human colon tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
4. IHC image of TMAH-01189 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

ELISA, WB, IHC

Monoclonal

Unconjugated

Recombinant Protein: Human TNFRSF17 Protein (1-54AA)

Human

608

Immunology