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CD7 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 19.69 kDa and the accession number is NP_006128.1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $600 | 7-10 days | |
100 μg | $1,390 | 7-10 days |
Biological Activity | Immobilized Recombinant Human SECTM1 / K12 Protein (His Tag) at 2μg/mL (100μL/well) can bind Recombinant Human CD7 Protein (ECD, His & AVI Tag), Biotinylated , the EC50 is 18-49 ng/mL. |
Description | CD7 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 19.69 kDa and the accession number is NP_006128.1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | NP_006128.1 |
Synonyms | TP41,Tp40,LEU-9,GP40,CD7 molecule |
Construction | A DNA sequence encoding the Human CD7 (NP_006128.1) (Met1-Pro180) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 26 |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Molecular Weight | 19.69 kDa (predicted); 34.46 kDa (reducing conditions) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell / B-cell interaction during early lymphoid development. |
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