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Anti-Peroxiredoxin 6 Antibody (5E148)

🥰Excellent
Catalog No. TMAY-01802

Anti-Peroxiredoxin 6 Antibody (5E148) is a Mouse antibody targeting Peroxiredoxin 6. Anti-Peroxiredoxin 6 Antibody (5E148) can be used in ELISA,IHC-P,IP.

Anti-Peroxiredoxin 6 Antibody (5E148)

Anti-Peroxiredoxin 6 Antibody (5E148)

🥰Excellent
Catalog No. TMAY-01802
Anti-Peroxiredoxin 6 Antibody (5E148) is a Mouse antibody targeting Peroxiredoxin 6. Anti-Peroxiredoxin 6 Antibody (5E148) can be used in ELISA,IHC-P,IP.
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Product Introduction

Bioactivity
Description
Anti-Peroxiredoxin 6 Antibody (5E148) is a Mouse antibody targeting Peroxiredoxin 6. Anti-Peroxiredoxin 6 Antibody (5E148) can be used in ELISA,IHC-P,IP.
Ig Type
Monoclonal Mouse IgG1
Clone
5E148
Reactivity
Human
Verified Activity
1. Immunochemical staining of human PRDX6 in human brain with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to neuroglia cells.

2. PRDX6 was immunoprecipitated using:

-Lane A:0.5 mg Hela Whole Cell Lysate.

-2 µL anti-PRDX6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-PRDX6 mouse monoclonal antibody, at 1:500 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 26 kDa.

-Observed band size: 26 kDa
Application
ELISA,IHC-P,IP
Recommended Dose
ELISA: 1:1000-1:2000; IHC-P: 1:50-1:200; IP: 4-6 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionThis antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Peroxiredoxin 6 / PRDX6 (rh Peroxiredoxin 6 / PRDX6; TMPY-02201; P30041; Met 1-Pro 224). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
Research BackgroundPRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Peroxiredoxin 6 (PRDX6) is involved in redox regulation of the cell and is thought to be protective against oxidant injury. Peroxiredoxin 6 (PRDX6) is a bifunctional protein with both glutathione peroxidase (GPx) and iPLA2 activities,which are concomitantly increased with the expression of PRDX6. PRDX6 promoted lung tumor growth in an in vivo allograft model.
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC/PE
Antibody Types Available
3
Antigen Details
Immunogen
Recombinant Human Peroxiredoxin 6 / PRDX6 protein (TMPY-02201)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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