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Anti-Peroxiredoxin 6 Antibody (5E148) is a Mouse antibody targeting Peroxiredoxin 6. Anti-Peroxiredoxin 6 Antibody (5E148) can be used in ELISA,IHC-P,IP.
Description | Anti-Peroxiredoxin 6 Antibody (5E148) is a Mouse antibody targeting Peroxiredoxin 6. Anti-Peroxiredoxin 6 Antibody (5E148) can be used in ELISA,IHC-P,IP. |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 5E148 |
Reactivity | Human |
Verified Activity | 1. Immunochemical staining of human PRDX6 in human brain with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to neuroglia cells. 2. PRDX6 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -2 µL anti-PRDX6 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PRDX6 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 26 kDa. -Observed band size: 26 kDa |
Application | ELISA,IHC-P,IP |
Recommended Dose | ELISA: 1:1000-1:2000; IHC-P: 1:50-1:200; IP: 4-6 μL/mg of lysate |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Peroxiredoxin 6 / PRDX6 (rh Peroxiredoxin 6 / PRDX6; TMPY-02201; P30041; Met 1-Pro 224). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
Research Background | PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Peroxiredoxin 6 (PRDX6) is involved in redox regulation of the cell and is thought to be protective against oxidant injury. Peroxiredoxin 6 (PRDX6) is a bifunctional protein with both glutathione peroxidase (GPx) and iPLA2 activities,which are concomitantly increased with the expression of PRDX6. PRDX6 promoted lung tumor growth in an in vivo allograft model. |
Conjucates | Unconjugated |
Others Formats | FITC/PE |
Antibody Types Available | 3 |
Immunogen | Recombinant Human Peroxiredoxin 6 / PRDX6 protein (TMPY-02201) |
Antigen Species | Human |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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