Complement Factor MASP3 Protein, Human, Recombinant (His)

Catalog No. TMPJ-00980 Copy Product Info

Complement Factor MASP3 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is P48740-2.

Complement Factor MASP3 Protein, Human, Recombinant (His)

Catalog No. TMPJ-00980

Copy Product Info

Complement Factor MASP3 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is P48740-2.

Pack Size	Price	USA Stock	Global Stock
5 μg	$71	7-10 days	7-10 days
10 μg	$108	7-10 days	7-10 days
20 μg	$166	7-10 days	7-10 days
50 μg	$293	7-10 days	7-10 days
100 μg	$476	7-10 days	7-10 days
200 μg	$756	7-10 days	7-10 days
500 μg	$1,390	7-10 days	7-10 days
1 mg	Inquiry	7-10 days	7-10 days

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Biological Activity	Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB). The specific activity is > 10000 pmol/min/µg. (QC Test) Immobilized Human MASP3, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-MASP3 Antibody, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA. (QC Test)
Description	Complement Factor MASP3 Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is P48740-2.
Species	Human
Expression System	HEK293 Cells
Tag	C-6xHis
Accession Number	P48740-2
Synonyms	Serine Protease 5,RaRF,PRSS5,MASP-1,MASP1,Mannose-Binding Lectin-Associated Serine Protease 1,CRARF1,CRARF,Complement Factor MASP-3,Complement Factor MASP3
Amino Acid	His20-Arg728
Construction	His20-Arg728
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Molecular Weight	31.10 kDa (predicted); 43-53 kDa (due to glycosylation)
Endotoxin	<1 EU/µg as determined by LAL test.
Formulation	Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, 10% Glycerol, pH 8.0.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	Proteins are shipped with blue ice.
Research Background	MASP3 is a member of the MASPs involved in mannan-binding lectin (MBL) complement pathway. The MBL pathway is initiated by the binding of MBL to specific carbohydrate structures found on the surface of a variety of microorganisms. Activation of the complement pathway via MBL is initiated by specific MASPs. Three MASPs have been identified and all have domain structures similar to those of C1r and C1s with a heavy chain (chain A) and a light chain (chain B). Chain A is composed of CUB1, EGF, CUB2, CCP1 and CCP2 while chain B corresponds to the catalytic domain found in many serine proteases. MASP1 and MASP3 are two alternatively spliced products of a single gene, which contain the same A chains but entirely different B chains. Distinct MASPs found in different MBL oligomers may have different biological activities. For example, MASP3, found together with MASP2, downregulates the C4 and C2 cleaving activity of MASP2. The protease activity of MASP3 is first revealed here using rhMASP3CD, which is inhibited by serine protease inhibitors such as Ecotin and AEBSF.