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Anti-Cathepsin A Antibody (5F9)
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Catalog No. TMAY-00888
Alias Ppgb, PPCA, cathepsin A, AU019505
Anti-Cathepsin A Antibody (5F9) is a Rabbit antibody targeting Cathepsin A. Anti-Cathepsin A Antibody (5F9) can be used in ELISA,ICC/IF.
Anti-Cathepsin A Antibody (5F9)
Anti-Cathepsin A Antibody (5F9)
😃Good
Catalog No. TMAY-00888Alias Ppgb, PPCA, cathepsin A, AU019505
Anti-Cathepsin A Antibody (5F9) is a Rabbit antibody targeting Cathepsin A. Anti-Cathepsin A Antibody (5F9) can be used in ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Cathepsin A Antibody (5F9) is a Rabbit antibody targeting Cathepsin A. Anti-Cathepsin A Antibody (5F9) can be used in ELISA,ICC/IF. |
| Alias | Ppgb, PPCA, cathepsin A, AU019505 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 5F9 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human CTSA in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CTSA monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm. |
| Application | ELISA,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human Cathepsin A / CTSA (rh Cathepsin A / CTSA; TMPY-00730; NP_001121167.1; Met1-Tyr480). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Lysosomal carboxypeptidase, cathepsin A (protective protein, CathA), is a component of the lysosomal multienzyme complex along with beta-galactosidase (GAL) and sialidase Neu1, where it activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation. Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities. It was recently identified in human platelets as deamidase. In vitro, it hydrolyzes a variety of bioactive peptide hormones including tachykinins, suggesting that extralysosomal cathepsin A plays a role in regulation of bioactive peptide functions. It is a member of the alpha/beta hydrolase fold family and has been suggested to share a common ancestral relationship with other alpha/beta hydrolase fold enzymes, such as cholinesterases. Cathepsin A defects are linked to multiple forms of Galactosialidosis with a combined secondary deficiency of beta-galactosidase and neuraminidase. Cathepsin A is a key molecule in the onset of galactosialidosis and also highlight the therapeutic acts in vivo as an endothelin-1-inactivating enzyme and strongly confirm a crucial role of this enzyme in effective elastic fiber formation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human Cathepsin A / CTSA protein (TMPY-00730) |
| Antigen Species | Human |
| Biology Area | Serine Proteases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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