Anti-Transferrin Receptor/TFRC Antibody (3E660) is a Rabbit antibody targeting Transferrin Receptor/TFRC. Anti-Transferrin Receptor/TFRC Antibody (3E660) can be used in ELISA,IP,WB.

Rabbit IgG

3E660

Human

Human Transferrin Receptor/TFR1/CD71

1. Anti-TFRC rabbit monoclonal antibody at 1:500 dilution.
-Lane A: Hela Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:84 kDa.
-Observed band size:95 kDa.
2. TFRC was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg MCF-7 Whole Cell Lysate.
-Lane C:0.5 mg HepG2 Whole Cell Lysate.
-Lane D:0.5 mg Hela Whole Cell Lysate.
-2 µL anti-TFRC rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.
-Primary antibody:
-Anti-TFRC rabbit monoclonal antibody, at 1:200 dilution.
-Secondary antibody:
-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.
-Developed using the odyssey technique.
-Performed under reducing conditions.
-Predicted band size: 84 kDa.
-Observed band size: 100 kDa.
3. Anti-TFRC rabbit monoclonal antibody at 1:500 dilution.
-Lane A: TFRC konckout Hela Whole Cell Lysate.
-Lane B: Hela Whole Cell lysate.
-Lysates/proteins at 20 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.
-Developed using the ECL technique.
-Performed under reducing conditions.
-Predicted band size:84 kDa.
-Observed band size:85 kDa(Validation Experiment)

WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 0.5-2 μL/mg of lysate

Monoclonal

Unconjugated

Recombinant Protein: Human Transferrin Receptor / TFRC / CD71 protein (TMPY-01873)

Human