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FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated

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Catalog No. TMPK-01678 Copy Product Info
FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is P05230-1.
FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human FGFR1 alpha (IIIc), His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 100.7 ng/ml determined by ELISA (QC Test).
Description
FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in E. coli. The accession number is P05230-1.
Species
Human
Expression System
E. coli
TagN-His-Avi
Accession NumberP05230-1
ConstructionPhe16-Asp155
Protein Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin< 1 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Research Background
Chemical Properties
Molecular Weight18.74 kDa (Predicted); 20-24 kDa (Reducing condition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | FGF-1 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight